| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC/IF/FC |
| Reactivity: |
Human/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-PIR51/RAD51AP1 (C-Term) is suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration: 4-6µg/ml.IF: Strong expression of the protein seen in the nuc |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
RAD51AP1 |
| Gene ID: |
10635 |
| Uniprot ID: |
R51A1_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_001124334.1; NP_006470 |
| Specificity: |
This antibody is expected to recognise both reported isoforms. |
| Immunogen Sequence: |
ARVKPLHPNATST |
| Post Translational Modifications | Sumoylation with SUMO2/3 by NSMCE2/MMS21 promotes stabilization, possibly by preventing ubiquitination. Sumoylation is required for alternative lengthening of telomeres (ALT) pathway. |
| Function | Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination. Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination. May also bind RNA.additional evidences are however required to confirm RNA-binding in vivo. |
| Protein Name | Rad51-Associated Protein 1Hsrad51ap1Rad51-Interacting Protein |
| Database Links | Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709603 |
| Cellular Localisation | ChromosomeNucleusTelomereColocalizes With Rad51 To Multiple Nuclear FociColocalizes With Dmc1 On Meiotic Chromatin |
| Alternative Antibody Names | Anti-Rad51-Associated Protein 1 antibodyAnti-Hsrad51ap1 antibodyAnti-Rad51-Interacting Protein antibodyAnti-RAD51AP1 antibodyAnti-PIR51 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
