• Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (STJ110322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (STJ110322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIP4K2B antibody (1-270) (STJ110322)

SKU:
STJ110322

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIP4K2B (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIP4K2B
Gene ID: 8396
Uniprot ID: PI42B_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PIP4K2B (NP_003550.1).
Immunogen Sequence: MSSNCTSTTAVAVAPLSASK TKTKKKHFVCQKVKLFRASE PILSVLMWGVNHTINELSNV PVPVMLMPDDFKAYSKIKVD NHLFNKENLPSRFKFKEYCP MVFRNLRERFGIDDQDYQNS VTRSAPINSDSQGRCGTRFL TTYDRRFVIKTVSSEDVAEM HNILKKYHQFIVECHGNTLL PQFLGMYRLTVDGVETYMVV TRNVFSHRLTVHRKYDLKGS TVAREASDKEKAKDLPTFK
Tissue Specificity Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.
Post Translational Modifications Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels. Phosphorylated on serine residues.
Function Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration. Its GTP-sensing activity is critical for metabolic adaptation. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3.
Protein Name Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Beta
1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Beta
Diphosphoinositide Kinase 2-Beta
Phosphatidylinositol 5-Phosphate 4-Kinase Type Ii Beta
Pi(5p 4-Kinase Type Ii Beta
Pip4kii-Beta
Ptdins(5p-4-Kinase Isoform 2-Beta
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-6811555
Reactome: R-HSA-6811558
Reactome: R-HSA-8847453
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cell Membrane
Nucleus
Cytoplasm
Associated With The Plasma Membrane And The Endoplasmic Reticulum
Alternative Antibody Names Anti-Phosphatidylinositol 5-Phosphate 4-Kinase Type-2 Beta antibody
Anti-1-Phosphatidylinositol 5-Phosphate 4-Kinase 2-Beta antibody
Anti-Diphosphoinositide Kinase 2-Beta antibody
Anti-Phosphatidylinositol 5-Phosphate 4-Kinase Type Ii Beta antibody
Anti-Pi(5p 4-Kinase Type Ii Beta antibody
Anti-Pip4kii-Beta antibody
Anti-Ptdins(5p-4-Kinase Isoform 2-Beta antibody
Anti-PIP4K2B antibody
Anti-PIP5K2B antibody

Information sourced from Uniprot.org

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