• Immunohistochemistry analysis of PIM1 in paraffin-embedded mouse spinal cord tissue using PIM1 Rabbit polyclonal antibody (STJ116142) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M PBS buffer (pH 7. 2±0. 2) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of PIM1 in paraffin-embedded rat testis tissue using PIM1 Rabbit polyclonal antibody (STJ116142) at a dilution of 1:100 (40x lens). Microwave antigen retrieval was performed with 0. 01 M PBS buffer (pH 7. 2±0. 2) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from mouse kidney, using PIM1 Rabbit polyclonal antibody (STJ116142) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using PIM1 Rabbit polyclonal antibody (STJ116142) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIM1 antibody (1-313) (STJ116142)

SKU:
STJ116142

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIM1 (1-313) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIM1
Gene ID: 5292
Uniprot ID: PIM1_HUMAN
Immunogen Region: 1-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PIM1 (NP_001230115.1).
Immunogen Sequence: MPHEPHEPLTPPFSALPDPA GAPSRRQSRQRPQLSSDSPS AFRASRSHSRNATRSHSHSH SPRHSLRHSPGSGSCGSSSG HRPCADILEVGMLLSKINSL AHLRAAPCNDLHATKLAPGK EKEPLESQYQVGPLLGSGGF GSVYSGIRVSDNLPVAIKHV EKDRISDWGELPNGTRVPME VVLLKKVSSGFSGVIRLLDW FERPDSFVLILERPEPVQDL FDFITERGALQEELARSFF
Tissue Specificity Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
Post Translational Modifications Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation. Ubiquitinated, leading to proteasomal degradation.
Function Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Acts as a positive regulator of mTORC1 signaling by mediating phosphorylation and inhibition of DEPDC5 component of the GATOR1 complex. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.
Protein Name Serine/Threonine-Protein Kinase Pim-1
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Isoform 2: Cell Membrane
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Pim-1 antibody
Anti-PIM1 antibody

Information sourced from Uniprot.org

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