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Anti-PIK3R4 antibody (1-330) (STJ118273)

Immunofluorescence analysis of NIH/3T3 cells using PIK3R4/VPS15 Rabbit polyclonal antibody (STJ118273) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PIK3R4/VPS15 antibody (STJ118273) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R4 antibody (1-330) (STJ118273)

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STJ118273

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-PIK3R4 (1-330) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:100 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PIK3R4
Gene ID:	30849
Uniprot ID:	PI3R4_HUMAN
Immunogen Region:	1-330
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4/VPS15 (NP_055417.1).
Immunogen Sequence:	MGNQLAGIAPSQILSVESYF SDIHDFEYDKSLGSTRFFKV ARAKHREGLVVVKVFAIQDP TLPLTSYKQELEELKIRLNS AQNCLPFQKASEKASEKAAM LFRQYVRDNLYDRISTRPFL NNIEKRWIAFQILTAVDQAH KSGVRHGDIKTENVMVTSWN WVLLTDFASFKPTYLPEDNP ADFNYFFDTSRRRTCYIAPE RFVDGGMFATELEYMRDPST PLVDLNSNQRTRGELKRAM

Tissue Specificity	Ubiquitously expressed.
Post Translational Modifications	Myristoylated. Probably autophosphorylated.
Function	Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Protein Name	Phosphoinositide 3-Kinase Regulatory Subunit 4 Pi3-Kinase Regulatory Subunit 4 Pi3-Kinase P150 Subunit Phosphoinositide 3-Kinase Adaptor Protein
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-1632852 Reactome: R-HSA-1660514 Reactome: R-HSA-1660516 Reactome: R-HSA-1660517 Reactome: R-HSA-168138 Reactome: R-HSA-5668599 Reactome: R-HSA-9679504 Reactome: R-HSA-9694676 Reactome: R-HSA-9705671 Reactome: R-HSA-983170
Cellular Localisation	Late Endosome Cytoplasmic Vesicle Autophagosome Membrane Lipid-Anchor As Component Of The Pi3k Complex I Localized To Pre-Autophagosome Structures As Component Of The Pi3k Complex Ii Localized Predominantly To Endosomes Localizes Also To Discrete Punctae Along The Ciliary Axoneme
Alternative Antibody Names	Anti-Phosphoinositide 3-Kinase Regulatory Subunit 4 antibody Anti-Pi3-Kinase Regulatory Subunit 4 antibody Anti-Pi3-Kinase P150 Subunit antibody Anti-Phosphoinositide 3-Kinase Adaptor Protein antibody Anti-PIK3R4 antibody Anti-VPS15 antibody

Information sourced from Uniprot.org

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