| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIK3R4 |
| Gene ID: | 30849 |
| Uniprot ID: | PI3R4_HUMAN |
| Immunogen Region: | 1-330 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human PIK3R4/VPS15 (NP_055417.1). |
| Immunogen Sequence: | MGNQLAGIAPSQILSVESYF SDIHDFEYDKSLGSTRFFKV ARAKHREGLVVVKVFAIQDP TLPLTSYKQELEELKIRLNS AQNCLPFQKASEKASEKAAM LFRQYVRDNLYDRISTRPFL NNIEKRWIAFQILTAVDQAH KSGVRHGDIKTENVMVTSWN WVLLTDFASFKPTYLPEDNP ADFNYFFDTSRRRTCYIAPE RFVDGGMFATELEYMRDPST PLVDLNSNQRTRGELKRAM |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Myristoylated. Probably autophosphorylated. |
| Function | Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2. |
| Protein Name | Phosphoinositide 3-Kinase Regulatory Subunit 4Pi3-Kinase Regulatory Subunit 4Pi3-Kinase P150 SubunitPhosphoinositide 3-Kinase Adaptor Protein |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1632852Reactome: R-HSA-1660514Reactome: R-HSA-1660516Reactome: R-HSA-1660517Reactome: R-HSA-168138Reactome: R-HSA-5668599Reactome: R-HSA-9679504Reactome: R-HSA-9694676Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | Late EndosomeCytoplasmic VesicleAutophagosomeMembraneLipid-AnchorAs Component Of The Pi3k Complex I Localized To Pre-Autophagosome StructuresAs Component Of The Pi3k Complex Ii Localized Predominantly To EndosomesLocalizes Also To Discrete Punctae Along The Ciliary Axoneme |
| Alternative Antibody Names | Anti-Phosphoinositide 3-Kinase Regulatory Subunit 4 antibodyAnti-Pi3-Kinase Regulatory Subunit 4 antibodyAnti-Pi3-Kinase P150 Subunit antibodyAnti-Phosphoinositide 3-Kinase Adaptor Protein antibodyAnti-PIK3R4 antibodyAnti-VPS15 antibody |
Information sourced from Uniprot.org
