• Western blot analysis of lysates from rat kidney, using PIGX Rabbit polyclonal antibody (STJ117655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIGX antibody (80-220) (STJ117655)

SKU:
STJ117655

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGX (80-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGX
Gene ID: 54965
Uniprot ID: PIGX_HUMAN
Immunogen Region: 80-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-220 of human PIGX (NP_060331.3).
Immunogen Sequence: LIKQDIPAGLYVDPYELASL RERNITEAVMVSENFDIEAP NYLSKESEVLIYARRDSQCI DCFQAFLPVHCRYHRPHSED GEASIVVNNPDLLMFCDQEF PILKCWAHSEVAAPCALENE DICQWNKMKYKSVYKNVILQ V
Post Translational Modifications N-glycosylated.
Function Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
Protein Name Phosphatidylinositol-Glycan Biosynthesis Class X Protein
Pig-X
Database Links Reactome: R-HSA-162710
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Phosphatidylinositol-Glycan Biosynthesis Class X Protein antibody
Anti-Pig-X antibody
Anti-PIGX antibody

Information sourced from Uniprot.org

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