• Immunohistochemistry analysis of PIGU in paraffin-embedded Rat ovary using PIGU Rabbit polyclonal antibody (STJ11100489) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PIGU in paraffin-embedded Human liver cancer using PIGU Rabbit polyclonal antibody (STJ11100489) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PIGU Rabbit polyclonal antibody (STJ11100489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIGU antibody (1-100) (STJ11100489)

SKU:
STJ11100489

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGU (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGU
Gene ID: 128869
Uniprot ID: PIGU_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PIGU (NP_536724.1).
Immunogen Sequence: MAAPLVLVLVVAVTVRAALF RSSLAEFISERVEVVSPLSS WKRVVEGLSLLDLGVSPYSG AVFHETPLIIYLFHFLIDYA ELVFMITDALTAIALYFAIQ
Function Component of the GPI transamidase complex, necessary for transfer of GPI to proteins. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.
Protein Name Phosphatidylinositol Glycan Anchor Biosynthesis Class U Protein
Cell Division Cycle Protein 91-Like 1
Protein Cdc91-Like 1
Gpi Transamidase Component Pig-U
Database Links Reactome: R-HSA-162791
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Phosphatidylinositol Glycan Anchor Biosynthesis Class U Protein antibody
Anti-Cell Division Cycle Protein 91-Like 1 antibody
Anti-Protein Cdc91-Like 1 antibody
Anti-Gpi Transamidase Component Pig-U antibody
Anti-PIGU antibody
Anti-CDC91L1 antibody
Anti-PSEC0205 antibody
Anti-UNQ3055 antibody
Anti-PRO9875 antibody

Information sourced from Uniprot.org

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