• Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using PIF4 Rabbit polyclonal antibody (STJ11103426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PIF4 antibody (300-400) (STJ11103426)

SKU:
STJ11103426

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Host: Rabbit
Applications: WB
Reactivity: A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIF4 (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of arabidopsis thaliana PIF4 (NP_565991.2).
Immunogen Sequence: IDYLKSLQLQLQVMWMGSGM AAAAASAPMMFPGVQPQQFI RQIQSPVQLPRFPVMDQSAI QNNPGLVCQNPVQNQIISDR FARYIGGFPHMQAATQMQPM E
Background Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm.

Information sourced from Uniprot.org

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