| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIAS4 (60-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIAS4 |
| Gene ID: |
51588 |
| Uniprot ID: |
PIAS4_HUMAN |
| Immunogen Region: |
60-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 60-260 of human PIAS4 (NP_056981.2). |
| Immunogen Sequence: |
ELYETRYAKKNSEPAPQPHR PLDPLTMHSTYDRAGAVPRT PLAGPNIDYPVLYGKYLNGL GRLPAKTLKPEVRLVKLPFF NMLDELLKPTELVPQNNEKL QESPCIFALTPRQVELIRNS RELQPGVKAVQVVLRICYSD TSCPQEDQYPPNIAVKVNHS YCSVPGYYPSNKPGVEPKRP CRPINLTHLMYLSSATNRIT V |
| Tissue Specificity | Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. |
| Post Translational Modifications | Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin. |
| Protein Name | E3 Sumo-Protein Ligase Pias4PiasyProtein Inhibitor Of Activated Stat Protein 4Protein Inhibitor Of Activated Stat Protein GammaPias-Gamma |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4615885Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusPml BodyColocalizes With Sumo1 And Tcf7l2/Tcf4 And Lef1 In A Subset Of Pml (Promyelocytic Leukemia) Nuclear Bodies |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias4 antibodyAnti-Piasy antibodyAnti-Protein Inhibitor Of Activated Stat Protein 4 antibodyAnti-Protein Inhibitor Of Activated Stat Protein Gamma antibodyAnti-Pias-Gamma antibodyAnti-PIAS4 antibodyAnti-PIASG antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
