| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIAS2 (420-621) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIAS2 |
| Gene ID: |
9063 |
| Uniprot ID: |
PIAS2_HUMAN |
| Immunogen Region: |
420-621 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 420-621 of human PIAS2 (NP_004662.2). |
| Immunogen Sequence: |
EDGSWCPMRPKKEAMKVSSQ PCTKIESSSVLSKPCSVTVA SEASKKKVDVIDLTIESSSD EEEDPPAKRKCIFMSETQSS PTKGVLMYQPSSVRVPSVTS VDPAAIPPSLTDYSVPFHHT PISSMSSDLPGLDFLSLIPV DPQYCPPMFLDSLTSPLTAS STSVTTTSSHESSTHVSSSS SRSETGVITSSGSNIPDIIS LD |
| Tissue Specificity | Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs. |
| Post Translational Modifications | Sumoylated. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'Lys-160'. |
| Protein Name | E3 Sumo-Protein Ligase Pias2Androgen Receptor-Interacting Protein 3Arip3Dab2-Interacting ProteinDipE3 Sumo-Protein Transferase Pias2Msx-Interacting Zinc Finger ProteinMiz1Pias-Ny ProteinProtein Inhibitor Of Activated Stat XProtein Inhibitor Of Activated Stat2 |
| Database Links | Reactome: R-HSA-3108214 O75928-1Reactome: R-HSA-3232118 O75928-1Reactome: R-HSA-3232142 O75928-1Reactome: R-HSA-3899300 O75928-1Reactome: R-HSA-4090294Reactome: R-HSA-4551638 O75928-2Reactome: R-HSA-5617472 |
| Cellular Localisation | Nucleus SpeckleNucleusPml BodyColocalizes At Least Partially With Promyelocytic Leukemia Nuclear Bodies (Pml Nbs)Colocalizes With Sumo1 In Nuclear Granules |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias2 antibodyAnti-Androgen Receptor-Interacting Protein 3 antibodyAnti-Arip3 antibodyAnti-Dab2-Interacting Protein antibodyAnti-Dip antibodyAnti-E3 Sumo-Protein Transferase Pias2 antibodyAnti-Msx-Interacting Zinc Finger Protein antibodyAnti-Miz1 antibodyAnti-Pias-Ny Protein antibodyAnti-Protein Inhibitor Of Activated Stat X antibodyAnti-Protein Inhibitor Of Activated Stat2 antibodyAnti-PIAS2 antibodyAnti-PIASX antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
