• Immunofluorescence analysis of NIH/3T3 cells using PI4KB Rabbit polyclonal antibody (STJ118844) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using PI4KB Rabbit polyclonal antibody (STJ118844) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Mouse thymus, using PI4KB antibody (STJ118844) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.

Anti-PI4KB antibody (560-801) (STJ118844)

SKU:
STJ118844

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PI4KB (560-801) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PI4KB
Gene ID: 5298
Uniprot ID: PI4KB_HUMAN
Immunogen Region: 560-801
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 560-801 of human PI4KB (NP_001185702).
Immunogen Sequence: AFQVLKQLQSIWEQERVPLW IKPYKILVISADSGMIEPVV NAVSIHQVKKQSQLSLLDYF LQEHGSYTTEAFLSAQRNFV QSCAGYCLVCYLLQVKDRHN GNILLDAEGHIIHIDFGFIL SSSPRNLGFETSAFKLTTEF VDVMGGLDGDMFNYYKMLML QGLIAARKHMDKVVQIVEIM QQGSQLPCFHGSSTIRNLKE RFHMSMTEEQLQLLVEQMVD GSMRSITTKLYDGFQYLTN
Tissue Specificity Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver. Expressed in the innear ear in the epithelium of the spinal organ of corti.
Function Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking. May play an important role in the inner ear development. (Microbial infection) Plays an essential role in Aichi virus RNA replication. Recruited by ACBD3 at the viral replication sites. (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.
Protein Name Phosphatidylinositol 4-Kinase Beta
Pi4k-Beta
Pi4kbeta
Ptdins 4-Kinase Beta
Npik
Pi4k92
Pi4kiii
Database Links Reactome: R-HSA-1660514
Cellular Localisation Endomembrane System
Mitochondrion Outer Membrane
Peripheral Membrane Protein
Rough Endoplasmic Reticulum Membrane
Golgi Apparatus
Golgi Apparatus Membrane
Cytoplasm
Perinuclear Region
Found In The Outer Membrane Of Mitochondria And Membranes Of The Rough Endoplasmic Reticulum
Recruited To The Golgi Complex By The Small Gtpase Arf To Stimulate The Synthesis Of Phosphatidylinositol 4
5-Bisphosphate (Pip2) On The Golgi Complex
Recruited To The Golgi Apparatus Membrane By Acbd3
Gga2 Is Also Involved In The Recruitment
Alternative Antibody Names Anti-Phosphatidylinositol 4-Kinase Beta antibody
Anti-Pi4k-Beta antibody
Anti-Pi4kbeta antibody
Anti-Ptdins 4-Kinase Beta antibody
Anti-Npik antibody
Anti-Pi4k92 antibody
Anti-Pi4kiii antibody
Anti-PI4KB antibody
Anti-PIK4CB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance