| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PI15 (128-141) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 33kDa band observed in lysates of cell line HeLa (calculated MW of 29.1kDa according to NP_056970.1). Recommended concentration: 0.3-1µg/ml. Preliminary testing was unsuccessful |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PI15 |
| Gene ID: | 51050 |
| Uniprot ID: | PI15_HUMAN |
| Immunogen Region: | 128-141 |
| Accession Number: | NP_056970.1 |
| Immunogen Sequence: | RTGRYRSILQLVKP |
| Post Translational Modifications | N-glycosylated. |
| Function | Serine protease inhibitor which displays weak inhibitory activity against trypsin. May play a role in facial patterning during embryonic development. |
| Protein Name | Peptidase Inhibitor 15Pi-1525 Kda Trypsin InhibitorP25tiCysteine-Rich Secretory Protein 8Crisp-8Sugarcrisp |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Peptidase Inhibitor 15 antibodyAnti-Pi-15 antibodyAnti-25 Kda Trypsin Inhibitor antibodyAnti-P25ti antibodyAnti-Cysteine-Rich Secretory Protein 8 antibodyAnti-Crisp-8 antibodyAnti-Sugarcrisp antibodyAnti-PI15 antibodyAnti-CRISP8 antibodyAnti-P25TI antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98597/382525/STJ119808_1__29834.1713150947.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SLC25A22 Rabbit polyclonal antibody (STJ119808) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
