• Immunohistochemistry analysis of paraffin-embedded mouse heart using PHPT1 antibody (STJ24983) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using PHPT1 antibody (STJ24983) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using PHPT1 antibody (STJ24983) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PHPT1 Rabbit polyclonal antibody (STJ24983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-PHPT1 antibody (1-125) (STJ24983)

SKU:
STJ24983

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHPT1 (1-125) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHPT1
Gene ID: 29085
Uniprot ID: PHP14_HUMAN
Immunogen Region: 1-125
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human PHPT1 (NP_054891.2).
Immunogen Sequence: MAVADLALIPDVDIDSDGVF KYVLIRVHSAPRSGAPAAES KEIVRGYKWAEYHADIYDKV SGDMQKQGCDCECLGGGRIS HQSQDKKIHVYGYSMAYGPA QHAISTEKIKAKYPDYEVTW ANDGY
Tissue Specificity Expressed abundantly in heart and skeletal muscle.
Function Exhibits phosphohistidine phosphatase activity.
Protein Name 14 Kda Phosphohistidine Phosphatase
Phosphohistidine Phosphatase 1
Phpt1
Protein Histidine Phosphatase
Php
Protein Janus-A Homolog
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-14 Kda Phosphohistidine Phosphatase antibody
Anti-Phosphohistidine Phosphatase 1 antibody
Anti-Phpt1 antibody
Anti-Protein Histidine Phosphatase antibody
Anti-Php antibody
Anti-Protein Janus-A Homolog antibody
Anti-PHPT1 antibody
Anti-PHP14 antibody
Anti-CGI-202 antibody
Anti-HSPC141 antibody

Information sourced from Uniprot.org

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