| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PHOX2B (Internal) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments in Human Brain (Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from pe |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PHOX2B |
| Gene ID: | 8929 |
| Uniprot ID: | PHX2B_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_003915.2 |
| Immunogen Sequence: | SRDDESKEAKSTDP |
| Function | Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. |
| Protein Name | Paired Mesoderm Homeobox Protein 2bNeuroblastoma PhoxNbphoxPhox2b Homeodomain ProteinPaired-Like Homeobox 2b |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Paired Mesoderm Homeobox Protein 2b antibodyAnti-Neuroblastoma Phox antibodyAnti-Nbphox antibodyAnti-Phox2b Homeodomain Protein antibodyAnti-Paired-Like Homeobox 2b antibodyAnti-PHOX2B antibodyAnti-PMX2B antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PHOX2B knockdown (KD) SH-SY5Y cells using [KD Validated]PHOX2B Rabbit polyclonal antibody (STJ11104636) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177280/368918/STJ11104636_1__71738.1713134397.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PHOX2B knockdown (KD) SH-SY5Y cells using [KD Validated]PHOX2B Rabbit polyclonal antibody (STJ11104636) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.")
