• Western blot analysis of extracts from HeLa cells, using Phospho-YWHAZ-S58 antibody (STJ22447). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of lysates from HeLa cells, using Phospho-YWHAZ-S58 Rabbit polyclonal antibody (STJ22447). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-YWHAZ-S58 antibody (STJ22447).
  • Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-YWHAZ-S58 antibody (STJ22447).

Anti-Phospho-YWHAZ-S58 antibody (STJ22447)

SKU:
STJ22447

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-YWHAZ-S58 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YWHAZ
Gene ID: 7534
Uniprot ID: 1433Z_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S58 of human YWHAZ
Immunogen Sequence: Email for sequence
Post Translational Modifications The delta, brain-specific form differs from the zeta form in being phosphorylated (Probable). Phosphorylation on Ser-184 by MAPK8.promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232.inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA.disrupts homodimerization and heterodimerization with YHAE and TP53.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
Protein Name 14-3-3 Protein Zeta/Delta
Protein Kinase C Inhibitor Protein 1
Kcip-1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-3769402
Reactome: R-HSA-392517
Reactome: R-HSA-430116
Reactome: R-HSA-450604
Reactome: R-HSA-512988
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-9013700
Reactome: R-HSA-9604323
Reactome: R-HSA-9614399
Reactome: R-HSA-9735871
Reactome: R-HSA-9755779
Cellular Localisation Cytoplasm
Melanosome
Located To Stage I To Stage Iv Melanosomes
Alternative Antibody Names Anti-14-3-3 Protein Zeta/Delta antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 antibody
Anti-YWHAZ antibody

Information sourced from Uniprot.org

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