• Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using WWOX (Phospho-Tyr33) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WWOX (Phospho-Tyr33) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-WWOX-Tyr33 antibody (18-67 aa) (STJ90971)

SKU:
STJ90971

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-WW domain-containing oxidoreductase-Tyr33 (18-67 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WWOX
Gene ID: 51741
Uniprot ID: WWOX_HUMAN
Immunogen Region: 18-67 aa
Specificity: Phospho-WWOX (Y33) Polyclonal Antibody detects endogenous levels of WWOX protein only when phosphorylated at Y33.
Immunogen: The antiserum was produced against synthesized peptide derived from the human WWOX around the phosphorylation site of Tyr33 at the amino acid range 18-67
Post Translational Modifications Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation. Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Function Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Protein Name Ww Domain-Containing Oxidoreductase
Fragile Site Fra16d Oxidoreductase
Short Chain Dehydrogenase/Reductase Family 41c Member 1
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-8866904
Reactome: R-HSA-8866907
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Golgi Apparatus
Lysosome
Partially Localizes To The Mitochondria
Translocates To The Nucleus Upon Genotoxic Stress Or Tnf Stimulation
Translocates To The Nucleus In Response To Tgfb1
Isoform 5 And Isoform 6 May Localize In The Nucleus
Localized To The Lysosome Probably Upon Binding To Vopp1
Alternative Antibody Names Anti-Ww Domain-Containing Oxidoreductase antibody
Anti-Fragile Site Fra16d Oxidoreductase antibody
Anti-Short Chain Dehydrogenase/Reductase Family 41c Member 1 antibody
Anti-WWOX antibody
Anti-FOR antibody
Anti-SDR41C1 antibody
Anti-WOX1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance