| Short Description : | Rabbit polyclonal anti-Phospho-Wee1-like protein kinase-Ser642 (597-646 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-1:2000ELISA 1:5000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WEE1 |
| Gene ID: | 7465 |
| Uniprot ID: | WEE1_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human WEE1 around the phosphorylation site of Ser642 at the amino acid range 597-646 |
| Immunogen Region: | 597-646 aa |
| Specificity: | Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642. |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Protein Name | Wee1-Like Protein KinaseWee1huWee1a Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69202Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-69656Reactome: R-HSA-75035Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase antibodyAnti-Wee1hu antibodyAnti-Wee1a Kinase antibodyAnti-WEE1 antibody |
Information sourced from Uniprot.org
