• Western blot analysis of lysates from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-WEE1-Ser642 antibody (597-646 aa) (STJ90550)

SKU:
STJ90550

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Wee1-like protein kinase-Ser642 (597-646 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WEE1
Gene ID: 7465
Uniprot ID: WEE1_HUMAN
Immunogen Region: 597-646 aa
Specificity: Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642.
Immunogen: The antiserum was produced against synthesized peptide derived from the human WEE1 around the phosphorylation site of Ser642 at the amino acid range 597-646
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Protein Name Wee1-Like Protein Kinase
Wee1hu
Wee1a Kinase
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-69202
Reactome: R-HSA-69273
Reactome: R-HSA-69478
Reactome: R-HSA-69656
Reactome: R-HSA-75035
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Wee1-Like Protein Kinase antibody
Anti-Wee1hu antibody
Anti-Wee1a Kinase antibody
Anti-WEE1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance