Anti-Phospho-WASF1-Tyr125 antibody (91-140 aa) (STJ90840)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human WAVE1 around the phosphorylation site of Tyr125 at the amino acid range 91-140
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Actin-binding protein WASF1-Tyr125 (91-140 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WASF1 |
| Gene ID | 8936 |
| Uniprot ID | WASF1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human WAVE1 around the phosphorylation site of Tyr125 at the amino acid range 91-140 |
| Immunogen Region | 91-140 aa |
| Specificity | Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125. |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12. |
| Function | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics. |
| Protein Name | Actin-Binding Protein Wasf1Protein Wave-1Verprolin Homology Domain-Containing Protein 1Wiskott-Aldrich Syndrome Protein Family Member 1Wasp Family Protein Member 1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-9013149Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCytoskeletonSynapseCell JunctionFocal AdhesionDot-Like Pattern In The CytoplasmConcentrated In Rac-Regulated Membrane-Ruffling AreasPartial Translocation To Focal Adhesion Sites Might Be Mediated By Interaction With Sorbs2In NeuronsColocalizes With Activated Ntrk2 After Bdnf Addition In Endocytic Sites Through The Association With Tmem108 |
| Alternative Antibody Names | Anti-Actin-Binding Protein Wasf1 antibodyAnti-Protein Wave-1 antibodyAnti-Verprolin Homology Domain-Containing Protein 1 antibodyAnti-Wiskott-Aldrich Syndrome Protein Family Member 1 antibodyAnti-Wasp Family Protein Member 1 antibodyAnti-WASF1 antibodyAnti-KIAA0269 antibodyAnti-SCAR1 antibodyAnti-WAVE1 antibody |
Information sourced from Uniprot.org