• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WAVE1 (Phospho-Tyr125) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using WAVE1 (Phospho-Tyr125) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-WAVE1 (Y125) Polyclonal Antibody

Anti-Phospho-WASF1-Tyr125 antibody (91-140 aa) (STJ90840)

SKU:
STJ90840

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Actin-binding protein WASF1-Tyr125 (91-140 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WASF1
Gene ID: 8936
Uniprot ID: WASF1_HUMAN
Immunogen Region: 91-140 aa
Specificity: Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.
Immunogen: The antiserum was produced against synthesized peptide derived from the human WAVE1 around the phosphorylation site of Tyr125 at the amino acid range 91-140
Post Translational Modifications Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.
Function Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics.
Protein Name Actin-Binding Protein Wasf1
Protein Wave-1
Verprolin Homology Domain-Containing Protein 1
Wiskott-Aldrich Syndrome Protein Family Member 1
Wasp Family Protein Member 1
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-4420097
Reactome: R-HSA-5663213
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Synapse
Cell Junction
Focal Adhesion
Dot-Like Pattern In The Cytoplasm
Concentrated In Rac-Regulated Membrane-Ruffling Areas
Partial Translocation To Focal Adhesion Sites Might Be Mediated By Interaction With Sorbs2
In Neurons
Colocalizes With Activated Ntrk2 After Bdnf Addition In Endocytic Sites Through The Association With Tmem108
Alternative Antibody Names Anti-Actin-Binding Protein Wasf1 antibody
Anti-Protein Wave-1 antibody
Anti-Verprolin Homology Domain-Containing Protein 1 antibody
Anti-Wiskott-Aldrich Syndrome Protein Family Member 1 antibody
Anti-Wasp Family Protein Member 1 antibody
Anti-WASF1 antibody
Anti-KIAA0269 antibody
Anti-SCAR1 antibody
Anti-WAVE1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance