Anti-Phospho-VHL-Ser68 antibody (34-83 aa) (STJ91273)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human VHL around the phosphorylation site of Ser68 at the amino acid range 34-83
General Information
| Short Description | Rabbit polyclonal anti-Phospho-von Hippel-Lindau disease tumor suppressor-Ser68 (34-83 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VHL |
| Gene ID | 7428 |
| Uniprot ID | VHL_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human VHL around the phosphorylation site of Ser68 at the amino acid range 34-83 |
| Immunogen Region | 34-83 aa |
| Specificity | Phospho-VHL (S68) Polyclonal Antibody detects endogenous levels of VHL protein only when phosphorylated at S68. |
Additional Info
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor SuppressorProtein G7Pvhl |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-3232142Reactome: R-HSA-8951664Reactome: R-HSA-9682706Reactome: R-HSA-9694686Reactome: R-HSA-9706019Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic ReticulumNucleusFound Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-AssociatedColocalizes With Adrb2 At The Cell MembraneIsoform 3: CytoplasmEqually Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Antibody Names | Anti-Von Hippel-Lindau Disease Tumor Suppressor antibodyAnti-Protein G7 antibodyAnti-Pvhl antibodyAnti-VHL antibody |
Information sourced from Uniprot.org