• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VHL-Ser68 antibody (34-83 aa) (STJ91273)

SKU:
STJ91273

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-von Hippel-Lindau disease tumor suppressor-Ser68 (34-83 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VHL
Gene ID: 7428
Uniprot ID: VHL_HUMAN
Immunogen Region: 34-83 aa
Specificity: Phospho-VHL (S68) Polyclonal Antibody detects endogenous levels of VHL protein only when phosphorylated at S68.
Immunogen: The antiserum was produced against synthesized peptide derived from the human VHL around the phosphorylation site of Ser68 at the amino acid range 34-83
Function Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.
Protein Name Von Hippel-Lindau Disease Tumor Suppressor
Protein G7
Pvhl
Database Links Reactome: R-HSA-1234176
Reactome: R-HSA-3232142
Reactome: R-HSA-8951664
Reactome: R-HSA-9682706
Reactome: R-HSA-9694686
Reactome: R-HSA-9706019
Reactome: R-HSA-983168
Cellular Localisation Isoform 1: Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum
Nucleus
Found Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-Associated
Colocalizes With Adrb2 At The Cell Membrane
Isoform 3: Cytoplasm
Equally Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated
Alternative Antibody Names Anti-Von Hippel-Lindau Disease Tumor Suppressor antibody
Anti-Protein G7 antibody
Anti-Pvhl antibody
Anti-VHL antibody

Information sourced from Uniprot.org

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