• Western blot analysis of lysates from HeLa cells treated with calyculinA 50ng/ml 30', using UBF (Phospho-Ser484) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using UBF (Phospho-Ser484) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-UBTF-Ser484 antibody (451-500 aa) (STJ90997)

SKU:
STJ90997

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nucleolar transcription factor 1-Ser484 (451-500 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBTF
Gene ID: 7343
Uniprot ID: UBF1_HUMAN
Immunogen Region: 451-500 aa
Specificity: Phospho-UBF (S484) Polyclonal Antibody detects endogenous levels of UBF protein only when phosphorylated at S484.
Immunogen: The antiserum was produced against synthesized peptide derived from the human UBF around the phosphorylation site of Ser484 at the amino acid range 451-500
Function Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Protein Name Nucleolar Transcription Factor 1
Autoantigen Nor-90
Upstream-Binding Factor 1
Ubf-1
Database Links Reactome: R-HSA-427413
Reactome: R-HSA-73728
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73863
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Nucleolar Transcription Factor 1 antibody
Anti-Autoantigen Nor-90 antibody
Anti-Upstream-Binding Factor 1 antibody
Anti-Ubf-1 antibody
Anti-UBTF antibody
Anti-UBF antibody
Anti-UBF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance