• Western blot analysis of lysates from PC-3 cells using Phospho-Ubiquitin-Ser65 Rabbit polyclonal antibody (STJ11105848) at 1:700 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone (30 uM) at 37 °C for 6 hours after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.
  • Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit polyclonal antibody (STJ11105848) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone (10 uM) (10 µM) at 37 °C for 6 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-Ubiquitin-Ser65 antibody (STJ11105848)

SKU:
STJ11105848

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA52
UBB
7311
7314
6233
RL40_HUMAN
UBB_HUMAN
RS27A_HUMAN
Specificity: A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Tissue Specificity
Alternative Antibody Names Anti-Ubiquitin-Ribosomal Protein El40 Fusion Protein antibody
Anti-Cep52 antibody
Anti-Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 Cleaved Into - Ubiquitin - Large Ribosomal Subunit Protein El40 antibody
Anti-60s Ribosomal Protein L40 antibody
Anti-Rpl40 antibody
Anti-UBA52 antibody
Anti-UBCEP2 antibody
Anti-Polyubiquitin-B Cleaved Into - Ubiquitin antibody
Anti-UBB antibody
Anti-Ubiquitin-Ribosomal Protein Es31 Fusion Protein antibody
Anti-Ubiquitin Carboxyl Extension Protein 80 Cleaved Into - Ubiquitin - Small Ribosomal Subunit Protein Es31 antibody
Anti-40s Ribosomal Protein S27a antibody
Anti-RPS27A antibody
Anti-UBA80 antibody
Anti-UBCEP1 antibody
Anti-Polyubiquitin-C Cleaved Into - Ubiquitin antibody
Anti-UBC antibody
Anti-Ubiquitin-Ribosomal Protein El40 Fusion Protein antibody
Anti-Cep52 antibody
Anti-Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 Cleaved Into - Ubiquitin - Large Ribosomal Subunit Protein El40 antibody
Anti-60s Ribosomal Protein L40 antibody
Anti-Rpl40 antibody
Anti-UBA52 antibody
Anti-UBCEP2 antibody
Anti-Polyubiquitin-B Cleaved Into - Ubiquitin antibody
Anti-UBB antibody
Anti-Ubiquitin-Ribosomal Protein Es31 Fusion Protein antibody
Anti-Ubiquitin Carboxyl Extension Protein 80 Cleaved Into - Ubiquitin - Small Ribosomal Subunit Protein Es31 antibody
Anti-40s Ribosomal Protein S27a antibody
Anti-RPS27A antibody
Anti-UBA80 antibody
Anti-UBCEP1 antibody
Anti-Polyubiquitin-C Cleaved Into - Ubiquitin antibody
Anti-UBC antibody
Anti-Ubiquitin-Ribosomal Protein El40 Fusion Protein antibody
Anti-Cep52 antibody
Anti-Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 Cleaved Into - Ubiquitin - Large Ribosomal Subunit Protein El40 antibody
Anti-60s Ribosomal Protein L40 antibody
Anti-Rpl40 antibody
Anti-UBA52 antibody
Anti-UBCEP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance