• Western blot analysis of lysates from PC-3 cells using Phospho-Ubiquitin-Ser65 Rabbit polyclonal antibody (STJ11105848) at 1:700 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone (30 uM) at 37 °C for 6 hours after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.
  • Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit polyclonal antibody (STJ11105848) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone (10 uM) (10 µM) at 37 °C for 6 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-Ubiquitin-Ser65 antibody (STJ11105848)

SKU:
STJ11105848

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Ubiquitin-Ser65 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Background Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Information sourced from Uniprot.org

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