| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Phospho-Trim24-S1043 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5806RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Trim24 |
| Gene ID: |
21848 |
| Uniprot ID: |
TIF1A_MOUSE |
| Immunogen: |
A synthetic phosphorylated peptide around S1043 of mouse Trim24 (NP_659542.3). |
| Immunogen Sequence: |
LKSTE |
| Tissue Specificity | Detected in embryonic and adult liver. Detected in zygote and throughout embryogenesis (at protein level). Detected in all adult tissues, with the highest expression level in testis. |
| Post Translational Modifications | Sumoylated. Phosphorylated at Ser-768 by ATM kinase induces ubiquitination and degradation during DNA damage. Undergoes ubiquitination-mediated degradation in response to DNA damage. |
| Function | Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, such as RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Required for normal transition from proliferating neonatal hepatocytes to quiescent adult hepatocytes. Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway. Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3. |
| Protein Name | Transcription Intermediary Factor 1-AlphaTif1-AlphaE3 Ubiquitin-Protein Ligase Trim24Ring-Type E3 Ubiquitin Transferase Tif1-AlphaTripartite Motif-Containing Protein 24 |
| Cellular Localisation | NucleusCytoplasmDetected In The Cytoplasm Of The ZygoteTranslocates Into The Pronucleus At The Time Of Genome ActivationColocalizes With Sites Of Active TranscriptionLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Transcription Intermediary Factor 1-Alpha antibodyAnti-Tif1-Alpha antibodyAnti-E3 Ubiquitin-Protein Ligase Trim24 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Tif1-Alpha antibodyAnti-Tripartite Motif-Containing Protein 24 antibodyAnti-Trim24 antibodyAnti-Tif1 antibodyAnti-Tif1a antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
