Anti-Phospho-TP53BP1-Ser25 antibody (10-59 aa) (STJ91300)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human 53BP1 around the phosphorylation site of Ser25 at the amino acid range 10-59
General Information
| Short Description | Rabbit polyclonal anti-Phospho-TP53-binding protein 1-Ser25 (10-59 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TP53BP1 |
| Gene ID | 7158 |
| Uniprot ID | TP53B_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human 53BP1 around the phosphorylation site of Ser25 at the amino acid range 10-59 |
| Immunogen Region | 10-59 aa |
| Specificity | Phospho-53BP1 (S25) Polyclonal Antibody detects endogenous levels of 53BP1 protein only when phosphorylated at S25. |
Additional Info
| Function | Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1. |
| Protein Name | Tp53-Binding Protein 153bp1P53-Binding Protein 1P53bp1 |
| Database Links | Reactome: R-HSA-3232118Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To The Nucleus In Absence Of Dna DamageFollowing Dna DamageRecruited To Sites Of Dna DamageSuch As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)Two Histone Marks That Are Present At Dsbs SitesAssociated With Kinetochores During Mitosis |
| Alternative Antibody Names | Anti-Tp53-Binding Protein 1 antibodyAnti-53bp1 antibodyAnti-P53-Binding Protein 1 antibodyAnti-P53bp1 antibodyAnti-TP53BP1 antibody |
Information sourced from Uniprot.org