| Short Description : | Rabbit polyclonal anti-Phospho-DNA topoisomerase 2-alpha-Ser1525 (1482-1531 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOP2A |
| Gene ID: | 7153 |
| Uniprot ID: | TOP2A_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human TOP2A around the phosphorylation site of Ser1525 at the amino acid range 1482-1531 |
| Immunogen Region: | 1482-1531 aa |
| Specificity: | Phospho-Topo II Alpha (S1525) Polyclonal Antibody detects endogenous levels of Topo II Alpha protein only when phosphorylated at S1525. |
| Post Translational Modifications | Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks. SUMOylated. |
| Function | Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. |
| Protein Name | Dna Topoisomerase 2-AlphaDna Topoisomerase Ii - Alpha Isozyme |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-4615885 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleolus |
| Alternative Antibody Names | Anti-Dna Topoisomerase 2-Alpha antibodyAnti-Dna Topoisomerase Ii - Alpha Isozyme antibodyAnti-TOP2A antibodyAnti-TOP2 antibody |
Information sourced from Uniprot.org
