Anti-Phospho-TIRAP-Tyr86 antibody (52-101 aa) (STJ91272)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human TIRAP around the phosphorylation site of Tyr86 at the amino acid range 52-101
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Toll/interleukin-1 receptor domain-containing adapter protein-Tyr86 (52-101 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TIRAP |
| Gene ID | 114609 |
| Uniprot ID | TIRAP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human TIRAP around the phosphorylation site of Tyr86 at the amino acid range 52-101 |
| Immunogen Region | 52-101 aa |
| Specificity | Phospho-TIRAP (Y86) Polyclonal Antibody detects endogenous levels of TIRAP protein only when phosphorylated at Y86. |
Additional Info
| Post Translational Modifications | Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation. |
| Function | Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha (TNF) and interleukin-6 (IL6). |
| Protein Name | Toll/Interleukin-1 Receptor Domain-Containing Adapter ProteinTir Domain-Containing Adapter ProteinAdaptor Protein WyattMyd88 Adapter-Like ProteinMyd88-2 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-166058Reactome: R-HSA-5602498Reactome: R-HSA-5603041 |
| Cellular Localisation | CytoplasmCell MembraneMembraneColocalizes With Dab2ip At The Plasma Membrane |
| Alternative Antibody Names | Anti-Toll/Interleukin-1 Receptor Domain-Containing Adapter Protein antibodyAnti-Tir Domain-Containing Adapter Protein antibodyAnti-Adaptor Protein Wyatt antibodyAnti-Myd88 Adapter-Like Protein antibodyAnti-Myd88-2 antibodyAnti-TIRAP antibodyAnti-MAL antibody |
Information sourced from Uniprot.org