• Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-TIFA-Thr9 antibody (STJ198347)

SKU:
STJ198347

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-TRAF-interacting protein with FHA domain-containing protein A-Thr9 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIFA
Gene ID: 92610
Uniprot ID: TIFA_HUMAN
Specificity: This antibody detects endogenous levels of TIFA (Phospho-Thr9) at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the human TIFA (Phospho-Thr9)
Function Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling.
Protein Name Traf-Interacting Protein With Fha Domain-Containing Protein A
Putative Mapk-Activating Protein Pm14
Putative Nf-Kappa-B-Activating Protein 20
Traf2-Binding Protein
Database Links Reactome: R-HSA-445989
Reactome: R-HSA-9645460
Cellular Localisation Cytoplasm
Colocalizes With Lysosomal Marker Lamp2 Following Homooligomerization And Subsequent Activation
Alternative Antibody Names Anti-Traf-Interacting Protein With Fha Domain-Containing Protein A antibody
Anti-Putative Mapk-Activating Protein Pm14 antibody
Anti-Putative Nf-Kappa-B-Activating Protein 20 antibody
Anti-Traf2-Binding Protein antibody
Anti-TIFA antibody
Anti-T2BP antibody

Information sourced from Uniprot.org

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