• Immunostaining of 14 DIV rat cortical neurons labeling synaptotagmin when phosphorylated at Thr202 (STJA0003791, 1:400, red) and PSD95 (green). The cortical neurons were fixed with methanol at-20C for 10 minutes. Photo courtesy of Gang Liu.
  • Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-SYT1-Thr202 antibody (STJA0003791)

SKU:
STJA0003791-100

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Host: Rabbit
Applications: WB/ICC
Reactivity: Rat/Bovine/Canine/Chicken/Human/Non-Human Primates/Mouse/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Synaptotagmin-Thr202 is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC 1:400
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Syt1
Gene ID: 25716
Uniprot ID: SYT1_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 conjugated to KLH.
Tissue Specificity Expressed in the brain (at protein level). Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.
Post Translational Modifications Glycosylated.
Function Calcium sensor that participates in triggering neurotransmitter release at the synapse. May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner.these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes. (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB).interaction is improved in the presence of gangliosides. BoNT/B toxin binds to the membrane proximal vesicular domain of Syt1 (residues 32-51). (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG).unlike the case with BoNT/B, interaction is not improved in the presence of gangliosides. BoNT/G toxin binds to the vesicular domain of Syt1 (residues 32-53).
Protein Name Synaptotagmin-1
Synaptotagmin I
Syti
P65
Database Links Reactome: R-RNO-181429
Reactome: -RNO-181430
Reactome: -RNO-210500
Reactome: -RNO-212676
Reactome: -RNO-264642
Reactome: -RNO-8856825
Reactome: -RNO-8856828
Reactome: -RNO-888590
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Membrane
Single-Pass Membrane Protein
Secretory Vesicle
Synaptic Vesicle Membrane
Chromaffin Granule Membrane
Cytoplasm
Alternative Antibody Names Anti-Synaptotagmin-1 antibody
Anti-Synaptotagmin I antibody
Anti-Syti antibody
Anti-P65 antibody
Anti-Syt1 antibody

Information sourced from Uniprot.org

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