• Western blot analysis of lysates from 293 cells treated with Sobital 0.4M 30', using Synaptotagmin (Phospho-Ser309) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Synaptotagmin (Phospho-Ser309) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody

Anti-Phospho-Synaptotagmin 1/2-Ser309/306 antibody (276-325 aa) (STJ90746)

SKU:
STJ90746

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Synaptotagmin-1 and Synaptotagmin-2-Ser309/306 (276-325 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYT1
SYT2
Gene ID: 6857
127833
Uniprot ID: SYT1_HUMAN
SYT2_HUMAN
Immunogen Region: 276-325 aa
Specificity: Phospho-Synaptotagmin 1/2 (S309/306) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1/2 protein only when phosphorylated at S309/306.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Synaptotagmin around the phosphorylation site of Ser309 at the amino acid range 276-325
Post Translational Modifications Glycosylated.
Function Calcium sensor that participates in triggering neurotransmitter release at the synapse. May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner.these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.
Protein Name Synaptotagmin-1
Synaptotagmin I
Syti
P65
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-5250958
Reactome: R-HSA-5250989
Reactome: R-HSA-6794361
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-888590
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle Membrane
Single-Pass Membrane Protein
Secretory Vesicle
Synaptic Vesicle Membrane
Chromaffin Granule Membrane
Cytoplasm
Alternative Antibody Names Anti-Synaptotagmin-1 antibody
Anti-Synaptotagmin I antibody
Anti-Syti antibody
Anti-P65 antibody
Anti-SYT1 antibody
Anti-SVP65 antibody
Anti-SYT antibody

Information sourced from Uniprot.org

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