• Immunofluorescence analysis of NIH/3T3 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 293 cells, using Syntaxin 1A (Phospho-Ser14) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-STX1A-Ser14 antibody (1-50 aa) (STJ90729)

SKU:
STJ90729

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Syntaxin-1A-Ser14 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX1A
Gene ID: 6804
Uniprot ID: STX1A_HUMAN
Immunogen Region: 1-50 aa
Specificity: Phospho-Syntaxin 1 (S14) Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein only when phosphorylated at S14.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Syntaxin 1A around the phosphorylation site of Ser14 at the amino acid range 1-50
Post Translational Modifications Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis.
Function Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).
Protein Name Syntaxin-1a
Neuron-Specific Antigen Hpc-1
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-264876
Reactome: R-HSA-422356
Reactome: R-HSA-449836
Reactome: R-HSA-5250971
Reactome: R-HSA-5682910
Reactome: R-HSA-6794361
Reactome: R-HSA-888590
Reactome: R-HSA-9609523
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Synapse
Synaptosome
Cell Membrane
Colocalizes With Kcnb1 At The Cell Membrane
Isoform 2: Secreted
Alternative Antibody Names Anti-Syntaxin-1a antibody
Anti-Neuron-Specific Antigen Hpc-1 antibody
Anti-STX1A antibody
Anti-STX1 antibody

Information sourced from Uniprot.org

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