Anti-Phospho-STK39-Ser309 antibody (277-326 aa) (STJ90959)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human STK39 around the phosphorylation site of Ser311 at the amino acid range 277-326
General Information
| Short Description | Rabbit polyclonal anti-Phospho-STE20/SPS1-related proline-alanine-rich protein kinase-Ser309 (277-326 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | STK39 |
| Gene ID | 27347 |
| Uniprot ID | STK39_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human STK39 around the phosphorylation site of Ser311 at the amino acid range 277-326 |
| Immunogen Region | 277-326 aa |
| Specificity | Phospho-SPAK (S311) Polyclonal Antibody detects endogenous levels of SPAK protein only when phosphorylated at S311. |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-231 by WNK kinases (WNK1, WNK2, WNK3 or WNK4) is required for activation. Autophosphorylation at Thr-231 positively regulates its activity. Phosphorylated at Ser-309 by PRKCQ. |
| Function | Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure. Specifically recognizes and binds proteins with a RFXV motif. Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity. Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx.simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities. Phosphorylates RELT. |
| Protein Name | Ste20/Sps1-Related Proline-Alanine-Rich Protein KinaseSte-20-Related KinaseDchtSerine/Threonine-Protein Kinase 39 |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusNucleus When Caspase-Cleaved |
| Alternative Antibody Names | Anti-Ste20/Sps1-Related Proline-Alanine-Rich Protein Kinase antibodyAnti-Ste-20-Related Kinase antibodyAnti-Dcht antibodyAnti-Serine/Threonine-Protein Kinase 39 antibodyAnti-STK39 antibodyAnti-PASK antibodyAnti-SPAK antibody |
Information sourced from Uniprot.org