• Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 polyclonal antibody (STJ11101025) at 1:1000 dilution. K-562 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of Phospho-STAT5-Y694 in paraffin-embedded mouse liver using Phospho-STAT5-Y694 Rabbit polyclonal antibody (STJ11101025) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 polyclonal antibody (STJ11101025) at 1:1000 dilution or STAT5B antibody (A12356). A-431 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of Phospho-STAT5-Y694 in paraffin-embedded human placenta using Phospho-STAT5-Y694 Rabbit polyclonal antibody (STJ11101025) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using Phospho-STAT5-Y694 antibody (STJ11101025) at dilution of 1:200 (40x lens).
  • Immunohistochemistry analysis of Phospho-STAT5-Y694 in paraffin-embedded rat heart using Phospho-STAT5-Y694 Rabbit polyclonal antibody (STJ11101025) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using Phospho-STAT5-Y694 antibody (STJ11101025) at dilution of 1:200 (40x lens).
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT5-Y694 Rabbit polyclonal antibody (STJ11101025) at 1:400 dilution. NIH/3T3 cells were treated by Pervanadate (1 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody (STJ11101025) at dilution of 1:200 (40x lens).

Anti-Phospho-STAT5A/STAT5B-Y694 antibody (STJ11101025)

SKU:
STJ11101025

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT5A
STAT5B
Gene ID: 6776
6777
Uniprot ID: STA5A_HUMAN
STA5B_HUMAN
Specificity: A synthetic phosphorylated peptide around Y694 of human STAT5 (NP_003143.2/NP_036580.2).
Immunogen Sequence: DGYVK
Tissue Specificity
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 5a antibody
Anti-STAT5A antibody
Anti-STAT5 antibody
Anti-Signal Transducer And Activator Of Transcription 5b antibody
Anti-STAT5B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance