| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Phospho-Stat1-Tyr-701 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M135 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
STAT1 |
| Gene ID: |
6772 |
| Uniprot ID: |
STAT1_HUMAN |
| Specificity: |
The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells. |
| Immunogen: |
Clone (M135) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 701 of human Stat1. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1. |
| Function | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine. |
| Protein Name | Signal Transducer And Activator Of Transcription 1-Alpha/BetaTranscription Factor Isgf-3 Components P91/P84 |
| Database Links | Reactome: R-HSA-1059683 P42224-1Reactome: R-HSA-1169408Reactome: R-HSA-1433557 P42224-1Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-2173795 P42224-1Reactome: R-HSA-6785807Reactome: R-HSA-877300 P42224-1Reactome: R-HSA-877312 P42224-1Reactome: R-HSA-8854691Reactome: R-HSA-8939902Reactome: R-HSA-8984722Reactome: R-HSA-8985947Reactome: R-HSA-9013508Reactome: R-HSA-9020956Reactome: R-HSA-9020958Reactome: R-HSA-909733Reactome: R-HSA-912694Reactome: R-HSA-9670439 P42224-1Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-9674555Reactome: R-HSA-9680350Reactome: R-HSA-9705462Reactome: R-HSA-9705671Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus Upon Tyrosine Phosphorylation And DimerizationIn Response To Ifn-Gamma And Signaling By Activated Fgfr1Fgfr2Fgfr3 Or Fgfr4Monomethylation At Lys-525 Is Required For Phosphorylation At Tyr-701 And Translocation Into The NucleusTranslocates Into The Nucleus In Response To Interferon-Beta Stimulation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 1-Alpha/Beta antibodyAnti-Transcription Factor Isgf-3 Components P91/P84 antibodyAnti-STAT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
