Anti-Phospho-SNCA-Ser129 antibody (STJA0003577)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Alpha synuclein-Ser129 for use in WB and IHC in Human, Mouse, Rat, Bovine, Canine and Non-Human Primates samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Non-Human Primates |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000IHC 1:200 |
| Formulation | 100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Snca |
| Gene ID | 29219 |
| Uniprot ID | SYUA_RAT |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH. |
Additional Info
| Tissue Specificity | Found only in brain (hippocampus, brainstem and cortex). Specifically expressed in neuronal cell bodies and synapses. |
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-Synuclein |
| Database Links | Reactome: R-RNO-9833482 |
| Cellular Localisation | CytoplasmMembraneNucleusSynapseSecretedCell ProjectionAxonMembrane-Bound In Dopaminergic NeuronsExpressed And Colocalized With Septin4 In Dopaminergic Axon TerminalsEspecially At The Varicosities |
| Alternative Antibody Names | Anti-Alpha-Synuclein antibodyAnti-Snca antibody |
Information sourced from Uniprot.org