• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SF3B1-Thr313 antibody (STJ196415)

SKU:
STJ196415

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Splicing factor 3B subunit 1-Thr313 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B1
Gene ID: 23451
Uniprot ID: SF3B1_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse SF3B1 (phospho-Thr313)
Immunogen: Synthesized phosho peptide around human SF3B1 (Thr313)
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Splicing Factor 3b Subunit 1
Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit
Sf3b155
Spliceosome-Associated Protein 155
Sap 155
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
During Mitosis
Transiently Dispersed From The Nuclear Speckles To The Cytoplasm
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 1 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibody
Anti-Sf3b155 antibody
Anti-Spliceosome-Associated Protein 155 antibody
Anti-Sap 155 antibody
Anti-SF3B1 antibody
Anti-SAP155 antibody

Information sourced from Uniprot.org

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