• Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SF3B1-Thr313 antibody (STJ196415)

SKU:
STJ196415

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Splicing factor 3B subunit 1-Thr313 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B1
Gene ID: 23451
Uniprot ID: SF3B1_HUMAN
Specificity: This antibody detects endogenous levels of Human Mouse SF3B1 (phospho-Thr313)
Immunogen: Synthesized phosho peptide around human SF3B1 (Thr313)
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92.
Protein Name Splicing Factor 3b Subunit 1
Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit
Sf3b155
Spliceosome-Associated Protein 155
Sap 155
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
During Mitosis
Transiently Dispersed From The Nuclear Speckles To The Cytoplasm
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 1 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibody
Anti-Sf3b155 antibody
Anti-Spliceosome-Associated Protein 155 antibody
Anti-Sap 155 antibody
Anti-SF3B1 antibody
Anti-SAP155 antibody

Information sourced from Uniprot.org

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