| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Splicing factor 1-Ser82 (48-97 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SF1 |
| Gene ID: | 7536 |
| Uniprot ID: | SF01_HUMAN |
| Immunogen Region: | 48-97 aa |
| Specificity: | Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human SF1 around the phosphorylation site of Ser82 at the amino acid range 48-97 |
| Post Translational Modifications | Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. |
| Function | Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. |
| Protein Name | Splicing Factor 1Mammalian Branch Point-Binding ProteinBbpMbbpTranscription Factor Zfm1Zinc Finger Gene In Men1 LocusZinc Finger Protein 162 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Splicing Factor 1 antibodyAnti-Mammalian Branch Point-Binding Protein antibodyAnti-Bbp antibodyAnti-Mbbp antibodyAnti-Transcription Factor Zfm1 antibodyAnti-Zinc Finger Gene In Men1 Locus antibodyAnti-Zinc Finger Protein 162 antibodyAnti-SF1 antibodyAnti-ZFM1 antibodyAnti-ZNF162 antibody |
Information sourced from Uniprot.org