• Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The lane on the right is blocked with the phospho peptide.
  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TIF-IA (Phospho-Ser649) Antibody

Anti-Phospho-RRN3-Ser649 antibody (602-651 aa) (STJ90994)

SKU:
STJ90994

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-RNA polymerase I-specific transcription initiation factor RRN3-Ser649 (602-651 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRN3
Gene ID: 54700
Uniprot ID: RRN3_HUMAN
Immunogen Region: 602-651 aa
Specificity: Phospho-Rrn3 (S649) Polyclonal Antibody detects endogenous levels of Rrn3 protein only when phosphorylated at S649.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TIF-IA around the phosphorylation site of Ser649 at the amino acid range 602-651
Function Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
Protein Name Rna Polymerase I-Specific Transcription Initiation Factor Rrn3
Transcription Initiation Factor Ia
Tif-Ia
Database Links Reactome: R-HSA-73762
Reactome: R-HSA-73772
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Rna Polymerase I-Specific Transcription Initiation Factor Rrn3 antibody
Anti-Transcription Initiation Factor Ia antibody
Anti-Tif-Ia antibody
Anti-RRN3 antibody
Anti-TIFIA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance