Anti-Phospho-RBL2-Thr642 antibody (STJA0010062)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human RBL2 (Phospho Thr642)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Retinoblastoma-like protein 2-Thr642 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBL2 |
| Gene ID | 5934 |
| Uniprot ID | RBL2_HUMAN |
| Immunogen | Synthesized peptide derived from human RBL2 (Phospho Thr642) |
| Specificity | This antibody detects endogenous levels of RBL2 (Phospho Thr642) Rabbit pAb at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. |
| Protein Name | Retinoblastoma-Like Protein 2130 Kda Retinoblastoma-Associated ProteinP130Retinoblastoma-Related Protein 2Rbr-2Prb2 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-6804114Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656Reactome: R-HSA-9617828 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Retinoblastoma-Like Protein 2 antibodyAnti-130 Kda Retinoblastoma-Associated Protein antibodyAnti-P130 antibodyAnti-Retinoblastoma-Related Protein 2 antibodyAnti-Rbr-2 antibodyAnti-Prb2 antibodyAnti-RBL2 antibodyAnti-RB2 antibody |
Information sourced from Uniprot.org