| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Retinoblastoma-like protein 2-Ser639 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RBL2 |
| Gene ID: | 5934 |
| Uniprot ID: | RBL2_HUMAN |
| Specificity: | This antibody detects endogenous levels of RBL2 (Phospho Ser639) Rabbit pAb at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human RBL2 (Phospho Ser639) |
| Post Translational Modifications | During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. |
| Protein Name | Retinoblastoma-Like Protein 2 130 Kda Retinoblastoma-Associated Protein P130 Retinoblastoma-Related Protein 2 Rbr-2 Prb2 |
| Database Links | Reactome: R-HSA-1362277 Reactome: R-HSA-1362300 Reactome: R-HSA-1538133 Reactome: R-HSA-6804114 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69231 Reactome: R-HSA-69656 Reactome: R-HSA-9617828 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Retinoblastoma-Like Protein 2 antibody Anti-130 Kda Retinoblastoma-Associated Protein antibody Anti-P130 antibody Anti-Retinoblastoma-Related Protein 2 antibody Anti-Rbr-2 antibody Anti-Prb2 antibody Anti-RBL2 antibody Anti-RB2 antibody |
Information sourced from Uniprot.org