| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-DNA endonuclease RBBP8-Ser664 is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RBBP8 |
| Gene ID: |
5932 |
| Uniprot ID: |
CTIP_HUMAN |
| Specificity: |
This antibody detects endogenous levels of RBBP8 (Phospho Ser664) Rabbit pAb at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human RBBP8 (Phospho Ser664) |
| Tissue Specificity | Expressed in ER-positive breast cancer lines, but tends to be down-regulated ER-negative cells (at protein level). |
| Post Translational Modifications | Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control. Phosphorylation at Thr-315, probably catalyzed by CDK2, is required for PIN1-binding, while phosphorylation at Ser-276 serves as a PIN1 isomerization site. Phosphorylation at Thr-315 is cell-cycle dependent. It steadily increases during S phase, peaks at late S/G2 phase, and drops at G1. Phosphorylation is not required for tetramerization. Binds to DNA more strongly when dephosphorylated. Ubiquitinated. Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteasomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control. Ubiquitinated by RNF138 at its N-terminus. Ubiquitinated through 'Lys-48' by the E3 CUL3-KLHL15 complex.this modification leads to proteasomal degradation. Ubiquitinated by the E3 FZR1/APC/C complex.this modification leads to proteasomal degradation. |
| Function | Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations. Binds preferentially to DNA Y-junctions and to DNA substrates with blocked ends and promotes intermolecular DNA bridging. |
| Protein Name | Dna Endonuclease Rbbp8Ctbp-Interacting ProteinCtipRetinoblastoma-Binding Protein 8Rbbp-8Retinoblastoma-Interacting Protein And Myosin-LikeRimSporulation In The Absence Of Spo11 Protein 2 HomologSae2 |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5685939Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-8953750Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusChromosomeAssociates With Sites Of Dna Damage In S/G2 PhaseUbiquitinated Rbbp8 Binds To Chromatin Following Dna Damage |
| Alternative Antibody Names | Anti-Dna Endonuclease Rbbp8 antibodyAnti-Ctbp-Interacting Protein antibodyAnti-Ctip antibodyAnti-Retinoblastoma-Binding Protein 8 antibodyAnti-Rbbp-8 antibodyAnti-Retinoblastoma-Interacting Protein And Myosin-Like antibodyAnti-Rim antibodyAnti-Sporulation In The Absence Of Spo11 Protein 2 Homolog antibodyAnti-Sae2 antibodyAnti-RBBP8 antibodyAnti-CTIP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
