• Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RAPGEF1-Tyr504 antibody (470-519 aa) (STJ90537)

SKU:
STJ90537

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Rap guanine nucleotide exchange factor 1-Tyr504 (470-519 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAPGEF1
Gene ID: 2889
Uniprot ID: RPGF1_HUMAN
Immunogen Region: 470-519 aa
Specificity: Phospho-C3G (Y504) Polyclonal Antibody detects endogenous levels of C3G protein only when phosphorylated at Y504.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RapGEF1 around the phosphorylation site of Tyr504 at the amino acid range 470-519
Post Translational Modifications Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
Function Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Protein Name Rap Guanine Nucleotide Exchange Factor 1
Crk Sh3-Binding Gnrp
Guanine Nucleotide-Releasing Factor 2
Protein C3g
Database Links Reactome: R-HSA-170968
Reactome: R-HSA-186763
Reactome: R-HSA-8875555
Reactome: R-HSA-9013423
Reactome: R-HSA-9027284
Reactome: R-HSA-912631
Cellular Localisation Early Endosome
Alternative Antibody Names Anti-Rap Guanine Nucleotide Exchange Factor 1 antibody
Anti-Crk Sh3-Binding Gnrp antibody
Anti-Guanine Nucleotide-Releasing Factor 2 antibody
Anti-Protein C3g antibody
Anti-RAPGEF1 antibody
Anti-GRF2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance