• Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RAD18-Ser403 antibody (STJ196394)

SKU:
STJ196394

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-E3 ubiquitin-protein ligase RAD18-Ser403 is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD18
Gene ID: 56852
Uniprot ID: RAD18_HUMAN
Specificity: This antibody detects endogenous levels of Human Rad18 (phospho-Ser403)
Immunogen: Synthesized phosho peptide around human Rad18 (Ser403)
Protein Name E3 Ubiquitin-Protein Ligase Rad18
Postreplication Repair Protein Rad18
Hhr18
Hrad18
Ring Finger Protein 73
Ring-Type E3 Ubiquitin Transferase Rad18
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-8866654
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Associates With Chromatin
Colocalizes With Slf1 In The Nucleus And To Centrosomes
Relocalizes With Slf1 To Nuclear Foci In Response To Dna Damage
Accumulates With The Slf1-Slf2 And Smc5-Smc6 Complexes At Replication-Coupled Dna Interstrand Repair And Dna Double-Strand Breaks (Dsbs) Sites On Chromatin In A Ubiquitin-Dependent Manner
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rad18 antibody
Anti-Postreplication Repair Protein Rad18 antibody
Anti-Hhr18 antibody
Anti-Hrad18 antibody
Anti-Ring Finger Protein 73 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rad18 antibody
Anti-RAD18 antibody
Anti-RNF73 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance