| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein phosphatase non-receptor type 6-Tyr536 (502-551 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PTPN6 |
| Gene ID: |
5777 |
| Uniprot ID: |
PTN6_HUMAN |
| Immunogen Region: |
502-551 aa |
| Specificity: |
Phospho-SH-PTP1 (Y536) Polyclonal Antibody detects endogenous levels of SH-PTP1 protein only when phosphorylated at Y536. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human SHP-1 around the phosphorylation site of Tyr536 at the amino acid range 502-551 |
| Post Translational Modifications | Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 by LYN enhances phosphatase activity. Phosphorylation at Thr-394 by TAOK3 leads to polyubiquitination and subsequent proteasomal degradation. Ubiquitinated after phosphorylation by TAOK3. Ubiquitinated by a cooperation between ITCH and WWP2 via 'Lys-27'-mediated polyubiquitin chains resulting in the reduction of its association with LCK. |
| Function | Tyrosine phosphatase enzyme that plays important roles in controlling immune signaling pathways and fundamental physiological processes such as hematopoiesis. Dephosphorylates and negatively regulate several receptor tyrosine kinases (RTKs) such as EGFR, PDGFR and FGFR, thereby modulating their signaling activities. When recruited to immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptors such as immunoglobulin-like transcript 2/LILRB1, programmed cell death protein 1/PDCD1, CD3D, CD22, CLEC12A and other receptors involved in immune regulation, initiates their dephosphorylation and subsequently inhibits downstream signaling events. Modulates the signaling of several cytokine receptors including IL-4 receptor. Additionally, targets multiple cytoplasmic signaling molecules including STING1, LCK or STAT1 among others involved in diverse cellular processes including modulation of T-cell activation or cGAS-STING signaling. Within the nucleus, negatively regulates the activity of some transcription factors such as NFAT5 via direct dephosphorylation. Acts also as a key transcriptional regulator of hepatic gluconeogenesis by controlling recruitment of RNA polymerase II to the PCK1 promoter together with STAT5A. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 6Hematopoietic Cell Protein-Tyrosine PhosphataseProtein-Tyrosine Phosphatase 1cPtp-1cProtein-Tyrosine Phosphatase Shp-1Sh-Ptp1 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-1433559Reactome: R-HSA-201556Reactome: R-HSA-210990Reactome: R-HSA-388841Reactome: R-HSA-389948Reactome: R-HSA-391160Reactome: R-HSA-432142Reactome: R-HSA-512988Reactome: R-HSA-5690714Reactome: R-HSA-6798695Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-9008059Reactome: R-HSA-909733Reactome: R-HSA-912526Reactome: R-HSA-912694Reactome: R-HSA-9705671Reactome: R-HSA-9725371Reactome: R-HSA-982772Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmNucleusIn NeuronsTranslocates Into The Nucleus After Treatment With Angiotensin IiShuttles Between The Cytoplasm And Nucleus Via Its Association With Pdpk1 |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 6 antibodyAnti-Hematopoietic Cell Protein-Tyrosine Phosphatase antibodyAnti-Protein-Tyrosine Phosphatase 1c antibodyAnti-Ptp-1c antibodyAnti-Protein-Tyrosine Phosphatase Shp-1 antibodyAnti-Sh-Ptp1 antibodyAnti-PTPN6 antibodyAnti-HCP antibodyAnti-PTP1C antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
