• Immunohistochemistry analysis of paraffin-embedded human brain, using PTP1B (Phospho-Ser50) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from COS7 cells treated with UV 30', using PTP1B (Phospho-Ser50) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of COLO cells using Phospho-PTP1B (S50) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PTPN1-Ser50 antibody (16-65 aa) (STJ90882)

SKU:
STJ90882

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein phosphatase non-receptor type 1-Ser50 (16-65 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN1
Gene ID: 5770
Uniprot ID: PTN1_HUMAN
Immunogen Region: 16-65 aa
Specificity: Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PTP1B around the phosphorylation site of Ser50 at the amino acid range 16-65
Post Translational Modifications Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Function Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 1
Protein-Tyrosine Phosphatase 1b
Ptp-1b
Database Links Reactome: R-HSA-354192
Reactome: R-HSA-6807004
Reactome: R-HSA-77387
Reactome: R-HSA-877312
Reactome: R-HSA-8849472
Reactome: R-HSA-9022699
Reactome: R-HSA-912694
Reactome: R-HSA-982772
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Interacts With Epha3 At The Cell Membrane
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibody
Anti-Protein-Tyrosine Phosphatase 1b antibody
Anti-Ptp-1b antibody
Anti-PTPN1 antibody
Anti-PTP1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance