• Western blot analysis of extracts of Jurkat cells, using Phospho-PTK2B-Y402 antibody (STJ22357) at 1:1000 dilution. Jurkat cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of lysates from Jurkat cells, using Phospho-PTK2B-Y402 Rabbit polyclonal antibody (STJ22357) at 1:1000 dilution. Jurkat cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-PTK2B-Y402 antibody (STJ22357).
  • Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-PTK2B-Y402 antibody (STJ22357).

Anti-Phospho-PTK2B-Y402 antibody (STJ22357)

SKU:
STJ22357

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-PTK2B-Y402 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTK2B
Gene ID: 2185
Uniprot ID: FAK2_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y402 of human PTK2B (NP_004094.3).
Immunogen Sequence: DIYAE
Tissue Specificity Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Post Translational Modifications Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration.this activation is indirect and may be mediated by production of reactive oxygen species (ROS). Tyr-402 is the major autophosphorylation site, but other kinases can also phosphorylate Tyr-402. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-402 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-579.Tyr-580 and Tyr-881. Phosphorylation at Tyr-881 is important for interaction with GRB2. Phosphorylated on tyrosine residues upon activation of FGR and PKC. Recruitment by NPHP1 to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. Phosphorylation by MYLK promotes ITGB2 activation and is thus essential to trigger neutrophil transmigration during lung injury. Dephosphorylated by PTPN12.
Function Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption.this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation.this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC.this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN.this requires both SRC and PTK2/PYK2.
Protein Name Protein-Tyrosine Kinase 2-Beta
Calcium-Dependent Tyrosine Kinase
Cadtk
Calcium-Regulated Non-Receptor Proline-Rich Tyrosine Kinase
Cell Adhesion Kinase Beta
Cak-Beta
Cakb
Focal Adhesion Kinase 2
Fadk 2
Proline-Rich Tyrosine Kinase 2
Related Adhesion Focal Tyrosine Kinase
Raftk
Database Links Reactome: R-HSA-391160
Reactome: R-HSA-4420097
Reactome: R-HSA-9013420
Reactome: R-HSA-9020558
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Focal Adhesion
Cell Projection
Lamellipodium
Cell Cortex
Nucleus
Interaction With Nphp1 Induces The Membrane-Association Of The Kinase
Colocalizes With Integrins At The Cell Periphery
Alternative Antibody Names Anti-Protein-Tyrosine Kinase 2-Beta antibody
Anti-Calcium-Dependent Tyrosine Kinase antibody
Anti-Cadtk antibody
Anti-Calcium-Regulated Non-Receptor Proline-Rich Tyrosine Kinase antibody
Anti-Cell Adhesion Kinase Beta antibody
Anti-Cak-Beta antibody
Anti-Cakb antibody
Anti-Focal Adhesion Kinase 2 antibody
Anti-Fadk 2 antibody
Anti-Proline-Rich Tyrosine Kinase 2 antibody
Anti-Related Adhesion Focal Tyrosine Kinase antibody
Anti-Raftk antibody
Anti-PTK2B antibody
Anti-FAK2 antibody
Anti-PYK2 antibody
Anti-RAFTK antibody

Information sourced from Uniprot.org

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