Western blot analysis of lysates from C6 cells, using Phospho-PKC (pan) (zeta) -T410 Rabbit pAb (STJ113486) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 ยฐC for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCI/PRKCQ/PRKCZ-T410 antibody (STJ113486)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ113486
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General Information

Short DescriptionRabbit polyclonal Phospho-PKC (pan) (zeta)-T410 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPRKCI
PRKCH
5584
5583
5582
KPCI_HUMAN
KPCL_HUMAN
KPCG_HUMAN
Immunogen SequenceTSTFC
SpecificityA synthetic phosphorylated peptide around T410 of human PRKCZ (NP_002735.3).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-Protein Kinase C Iota Type antibody
Anti-Atypical Protein Kinase C-Lambda/Iota antibody
Anti-Prkc-Lambda/Iota antibody
Anti-Apkc-Lambda/Iota antibody
Anti-Npkc-Iota antibody
Anti-PRKCI antibody
Anti-DXS1179E antibody
Anti-Protein Kinase C Eta Type antibody
Anti-Pkc-L antibody
Anti-Npkc-Eta antibody
Anti-PRKCH antibody
Anti-PKCL antibody
Anti-PRKCL antibody
Anti-Protein Kinase C Gamma Type antibody
Anti-Pkc-Gamma antibody
Anti-PRKCG antibody
Anti-PKCG antibody
Anti-Protein Kinase C Theta Type antibody
Anti-Npkc-Theta antibody
Anti-PRKCQ antibody
Anti-PRKCT antibody
Anti-Protein Kinase C Alpha Type antibody
Anti-Pkc-A antibody
Anti-Pkc-Alpha antibody
Anti-PRKCA antibody
Anti-PKCA antibody
Anti-PRKACA antibody
Anti-Protein Kinase C Delta Type antibody
Anti-Tyrosine-Protein Kinase Prkcd antibody
Anti-Npkc-Delta Cleaved Into - Protein Kinase C Delta Type Regulatory Subunit - Protein Kinase C Delta Type Catalytic Subunit antibody
Anti-Sphingosine-Dependent Protein Kinase-1 antibody
Anti-Sdk1 antibody
Anti-PRKCD antibody
Anti-PKCD antibody
Anti-Protein Kinase C Epsilon Type antibody
Anti-Npkc-Epsilon antibody
Anti-PRKCE antibody
Anti-PKCE antibody
Anti-Protein Kinase C Zeta Type antibody
Anti-Npkc-Zeta antibody
Anti-PRKCZ antibody
Anti-PKC2 antibody
Anti-Protein Kinase C Beta Type antibody
Anti-Pkc-B antibody
Anti-Pkc-Beta antibody
Anti-PRKCB antibody
Anti-PKCB antibody
Anti-PRKCB1 antibody
Anti-Protein Kinase C Iota Type antibody
Anti-Atypical Protein Kinase C-Lambda/Iota antibody
Anti-Prkc-Lambda/Iota antibody
Anti-Apkc-Lambda/Iota antibody
Anti-Npkc-Iota antibody
Anti-PRKCI antibody
Anti-DXS1179E antibody
Anti-Protein Kinase C Eta Type antibody
Anti-Pkc-L antibody
Anti-Npkc-Eta antibody
Anti-PRKCH antibody
Anti-PKCL antibody

Information sourced from Uniprot.org

Citations

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