• Western blot analysis of extracts of various cells, using Phospho-PPP2CA/PPP2CB-Y307 antibody (STJ11101061) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit polyclonal antibody (STJ11101061) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PPP2CA/PPP2CB-Y307 antibody (STJ11101061)

SKU:
STJ11101061

£129.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2CA
PPP2CB
Gene ID: 5515
5516
Uniprot ID: PP2AA_HUMAN
PP2AB_HUMAN
Specificity: A synthetic phosphorylated peptide around Y307 of human PPP2CA/PPP2CBPPP2CB (NP_001009552.1).
Immunogen Sequence: PDYFL
Tissue Specificity
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody
Anti-Pp2a-Alpha antibody
Anti-Replication Protein C antibody
Anti-Rp-C antibody
Anti-PPP2CA antibody
Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Beta Isoform antibody
Anti-Pp2a-Beta antibody
Anti-PPP2CB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance