• Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr75 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-PPP1R1B-Thr75 antibody (STJA0003608)

SKU:
STJA0003608-100

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DARPP-32-Thr75 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ppp1r1b
Gene ID: 360616
Uniprot ID: PPR1B_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH.
Post Translational Modifications Phosphorylation of Thr-34 is required for activity. Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Function Inhibitor of protein-phosphatase 1.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1b
Darpp-32
Dopamine- And Camp-Regulated Neuronal Phosphoprotein
Database Links Reactome: R-RNO-180024
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 1b antibody
Anti-Darpp-32 antibody
Anti-Dopamine- And Camp-Regulated Neuronal Phosphoprotein antibody
Anti-Ppp1r1b antibody

Information sourced from Uniprot.org

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